transam transcription factor assay kits for stat family  (Active Motif)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

doi: 10.1111/jcmm.14290

Figure Lengend Snippet: ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and JAK2/STAT3 signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments

Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

Techniques: Knockdown, Western Blot, Fractionation, Immunostaining, Activation Assay, Microscopy

Journal: Journal of Cellular and Molecular Medicine

Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

doi: 10.1111/jcmm.14290

Figure Lengend Snippet: Stattic, a STAT3 inhibitor, attenuates IL‐8 expression in colon cancer cells in a ZNF143‐dependent manner. (A) Cells were treated with 5 μmol/L Stattic for the indicated time periods and harvested to determine STAT3 phosphorylation by immunoblotting. (B, C) Cells were treated with 0, 1, 2.5, 5, and 10 μmol/L Stattic for 6 hours (B) or 24 hours (C) and harvested to determine STAT3 phosphorylation (B) or IL‐8 expression (C) by immunoblotting. Results shown are representative of at least three independent experiments

Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

Techniques: Expressing, Phospho-proteomics, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

doi: 10.1111/jcmm.14290

Figure Lengend Snippet: Increased IL‐8 contributes to STAT3 activation and IL‐8 expression through its receptor, CXCR2, in colon cancer cells. (A, B) Cells were harvested and analysed for mRNA expression of IL‐8 by reverse transcription (RT)‐polymerase chain reaction (PCR) (A) or real‐time PCR (B). (C) Cells were harvested, and the expression of signalling pathway components was determined by immunoblotting. Cells were treated with SB225002, an inhibitor of CXCR1, Stattic, or DMSO (vehicle) for 6 hours prior to harvesting. Results shown are representative of at least three independent experiments. Data are expressed as means ± S.E. of at least three independent experiments. Statistical significance was assessed using unpaired Student's t tests (*** P < 0.0001 and * P < 0.002)

Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

Techniques: Activation Assay, Expressing, Reverse Transcription, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Western Blot